Microbial abundance and community composition in biofilms on in-pipe sensors in a drinking water distribution system
نویسندگان
چکیده
Collecting biofilm samples from drinking water distribution systems (DWDSs) is challenging due to limited access the pipes during regular operations. We report here analysis of microbial communities in and collected sensors installed a DWDS where monochloramine used as residual disinfectant. A total 52 14 bulk were 17 pipe sections representing different ages. Prokaryotic genome copies (bacterial archaeal 16S rRNA genes, Mycobacterium spp., ammonia-oxidizing bacteria (AOB), cyanobacteria) quantified with droplet digital PCR, which revealed abundance these genes both samples. gene sequencing was carried out for subset (12 four sites). AOB species dominant low age sufficient monochloramine, whereas Nitrospira (nitrite-oxidizing bacteria) dominated higher depleted level, suggesting occurrence nitrification studied DWDS. The present study provides novel information on identity prokaryotes biofilms full-scale operational
منابع مشابه
Characterisation of the Physical Composition and Microbial Community Structure of Biofilms within a Model Full-Scale Drinking Water Distribution System
Within drinking water distribution systems (DWDS), microorganisms form multi-species biofilms on internal pipe surfaces. A matrix of extracellular polymeric substances (EPS) is produced by the attached community and provides structure and stability for the biofilm. If the EPS adhesive strength deteriorates or is overcome by external shear forces, biofilm is mobilised into the water potentially ...
متن کاملStructure and microbial diversity of biofilms on different pipe materials of a model drinking water distribution systems
The experiment was conducted in three model drinking water distribution systems (DWDSs) made of unplasticized polyvinyl chloride (PVC), silane cross-linked polyethylene (PEX) and high density polyethylene (HDPE) pipes to which tap water was introduced. After 2 years of system operation, microbial communities in the DWDSs were characterized with scanning electron microscopy, heterotrophic plate ...
متن کاملDetection of Escherichia coli in biofilms from pipe samples and coupons in drinking water distribution networks.
Fluorescence in situ hybridization (FISH) was used for direct detection of Escherichia coli on pipe surfaces and coupons in drinking water distribution networks. Old cast iron main pipes were removed from water distribution networks in France, England, Portugal, and Latvia, and E. coli was analyzed in the biofilm. In addition, 44 flat coupons made of cast iron, polyvinyl chloride, or stainless ...
متن کاملa study on rate making and required reserves determination in reinsurance market: a simulation
reinsurance is widely recognized as an important instrument in the capital management of an insurance company as well as its risk management tool. this thesis is intended to determine premium rates for different types of reinsurance policies. also, given the fact that the reinsurance coverage of every company depends upon its reserves, so different types of reserves and the method of their calc...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Science of The Total Environment
سال: 2021
ISSN: ['0048-9697', '1879-1026']
DOI: https://doi.org/10.1016/j.scitotenv.2020.142314